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Item #:
145280647
EGTA 0.5M, pH 8.0, 100mL. One Bottle of 100mL.
Item #:
145280647
IMP 32
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What Stands Out
High Purity
EGTA 0.5M offers superior purity for consistent results, making it ideal for sensitive biochemical applications and research needs.
Optimal pH Control
Maintained at pH 8.0, this solution provides excellent buffering capacity, ensuring stability in various experimental conditions for reliable outcomes.
Convenient Packaging
Supplied in a ready-to-use 100mL bottle, it simplifies handling and storage, catering to both laboratory and field applications for immediate usability.
Product Details
| Item Weight | 0.25 lbs (110 grams) |
Who Should Buy?
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Biochemists
Ideal for biochemists requiring a stable chelating agent for calcium ions in various biochemical experiments.
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Molecular Biology
Useful for molecular biologists needing to control metal ion concentrations in DNA and protein studies.
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Analytical Chemistry
Perfect for analytical chemists engaged in experiments where EDTA or similar agents are beneficial for metal ion analysis.
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General Consumers
Not suitable for general consumers without laboratory experience or specific research needs involving chelating agents.
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Non-Scientific Uses
Not recommended for applications outside of scientific research or academic disciplines where specialized knowledge is required.
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Simple Water Testing
Not the best choice for casual users needing quick water testing solutions without advanced chemical properties.
Product Description
EGTA 0.5M, pH 8.0, 100mL. One Bottle of 100mL.
Have any Query? Chat with us
Customer Questions & Answers
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Question:
What is EGTA and its primary use in laboratories?
Answer: EGTA (ethylene glycol bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid) is a chelating agent that effectively binds to calcium ions, making it crucial in various biochemical and molecular biology applications. It's primarily used to study calcium-dependent processes and regulate enzyme activity by buffering calcium levels. For instance, if you're conducting experiments on cellular signaling pathways influenced by calcium, using EGTA can help create an environment where calcium concentration is controlled. -
Question:
How should EGTA 0.5M pH 8.0 be stored for optimal performance?
Answer: To maintain the integrity of EGTA 0.5M, it should be stored in a cool, dark place, ideally at a temperature between 2-8°C. Keeping it away from direct sunlight and high temperatures prevents degradation, ensuring its effectiveness for your experiments. For example, if you’re preparing stock solutions for enzyme assays, proper storage will help maintain the chelator’s stability, thereby enhancing experimental accuracy. -
Question:
Can EGTA 0.5M be used for cell culture applications?
Answer: Yes, EGTA 0.5M can be beneficial in cell culture applications, particularly for dissociating cells from tissues. When used in trypsin-free protocols, EGTA helps to break down calcium-dependent cell adhesion, facilitating the harvesting of cells without damaging them. This is especially useful in experiments requiring healthy, viable cells to study growth or metabolic processes. -
Question:
What are the safety precautions when handling EGTA?
Answer: When handling EGTA, it is essential to wear appropriate laboratory gloves, goggles, and a lab coat to prevent skin contact and inhalation of dust. While EGTA is generally considered low in toxicity, ensuring safe handling minimizes any risk. In case of exposure, rinsing with water is recommended. This is critical in a laboratory setting, where safety protocols must be adhered to for all reagents. -
Question:
Is EGTA suitable for use in biological assays?
Answer: EGTA is indeed suitable for various biological assays as it can effectively manipulate calcium concentrations in experimental setups. By chelating calcium ions, it can prevent unintended activation of calcium-dependent enzymes or pathways, providing clearer insight into the mechanisms being studied. For example, if you're assessing enzyme kinetics that is influenced by calcium levels, EGTA allows for controlled experimentation. -
Question:
How does the pH level of EGTA 0.5M affect its performance?
Answer: The pH level of EGTA 0.5M, specifically pH 8.0, is optimized for maximum chelation efficiency. At this pH, EGTA effectively binds to calcium ions while minimizing hydrolysis reactions that can occur at lower pH levels. This balance ensures that the chelating agent remains active and effective in biological research, especially in processes that are sensitive to pH changes, making it crucial for accurate experimental outcomes. -
Question:
What types of experiments benefit from using EGTA?
Answer: EGTA is particularly beneficial in experiments involving protein purification, cellular signaling studies, and enzyme assays where calcium ions' presence needs to be controlled. For instance, if you're investigating the role of calcium in neurotransmitter release, using EGTA to sequester calcium ions can help clarify its impact without confounding variables. This allows researchers to draw more accurate conclusions about their studies. -
Question:
Can EGTA cause any issues in certain experimental setups?
Answer: While EGTA is valuable, it can interfere with experiments that require calcium as a cofactor for enzyme activity. In such cases, using too much EGTA can lead to inaccurate or misleading results. It's crucial to evaluate whether calcium ions are needed for your specific research application before incorporating EGTA. For example, in studies focusing on calcium-dependent kinases, its use would require careful consideration to avoid masking the true effects of calcium. -
Question:
What is the shelf life of EGTA 0.5M, pH 8.0?
Answer: The shelf life of EGTA 0.5M, pH 8.0 can vary based on storage conditions but typically lasts several months to a year when kept properly. It's recommended to check for any precipitates or changes in color before use as indicators of degradation. Always consult the manufacturer's specifications for more accurate information to ensure experimental reliability. As with all reagents, ensuring freshness is key to obtaining reproducible results. -
Question:
Where can I buy EGTA 0.5M, pH 8.0, 100mL?
Answer: You can buy EGTA 0.5M, pH 8.0, 100mL from Ubuy. This site provides a user-friendly platform for purchasing such laboratory reagents, offering a streamlined shopping experience tailored for researchers and scientists needing reliable chemical solutions. Explore their inventory to find this specific product that meets your laboratory's requirements.
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Features & Benefits
- High-quality EGTA solution at 0.5M concentration.
- pH 8.0 for optimal performance in research.
- Each bottle contains 100mL for extensive experimental use.
- Produced in a certified USA facility ensuring strict quality controls.
- Meets Good Manufacturing Practices and clean room standards.
- Designed exclusively for research applications, not for clinical use.
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