ProSignalTM Femto ECL Reagent, Low-femtogram, 1000cm2, 100ml Kit/Unit
IMP 585
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ProSignal Femto offers attomole sensitivity and an 8+ hour quantitative signal that allows you to detect bands that cannot be visualized with other Western blotting ECL detection reagents.
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Product Details
| Item Weight | 1 lbs (450 grams) |
Who Should Buy?
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Research Laboratories
Ideal for laboratories conducting sensitive protein detection and analysis requiring low-femtogram sensitivity levels.
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Clinical Diagnostics
Useful in clinical settings where accurate measurements of low-abundance biomarkers are critical for patient diagnosis.
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Biotechnology Companies
Beneficial for biotech firms focusing on developing assays that require extreme sensitivity and specificity in detection methods.
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Basic Education
Not suitable for educational purposes where low sensitivity requirements and high-volume testing are not necessary.
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Cost-Conscious Users
May be prohibitively expensive for users or organizations with tight budgets looking for basic detection solutions.
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High-Volume Production
Inappropriate for environments needing rapid, high-throughput testing that does not require the reagent's extreme sensitivity.
Product Description
ProSignalTM Femto ECL Reagent, Low-femtogram, 1000cm2, 100ml Kit/Unit
Customer Questions & Answers
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Question:
What is ProSignal Femto ECL Reagent used for?
Answer: ProSignal Femto ECL Reagent is designed for use in chemiluminescent Western blotting applications. It provides sensitive detection of low-abundance proteins, making it ideal for researchers who need to analyze small quantities of targets in complex samples. This reagent enhances signal detection, allowing for clearer, more defined results that are essential for accurate scientific analysis. It is especially useful in fields like biochemistry and molecular biology where high sensitivity is crucial. -
Question:
How does ProSignal Femto ECL Reagent compare to other detection methods?
Answer: ProSignal Femto ECL Reagent outperforms many traditional detection methods, such as colorimetric or fluorescent techniques, in terms of sensitivity. Its low-femtogram detection limit allows scientists to visualize proteins that might otherwise go undetected. This enhanced sensitivity is particularly beneficial when working with rare proteins or when sample sizes are limited, ultimately leading to more reliable data in experiments and studies. -
Question:
What types of samples can be used with ProSignal Femto ECL Reagent?
Answer: ProSignal Femto ECL Reagent can be used with a variety of sample types, including cell lysates, serum, and tissue extracts. Its compatibility with diverse biological samples allows for flexibility in experimental design. Researchers often utilize it for studying disease markers, protein expression, and interaction studies, making it an invaluable tool in biological research and diagnostics. -
Question:
How should I store ProSignal Femto ECL Reagent?
Answer: ProSignal Femto ECL Reagent should be stored at -20°C to maintain its efficacy. Proper storage is crucial to prevent degradation and ensure consistent performance over time. For best results, it’s recommended to avoid repeated freeze-thaw cycles. When prepared for experiments, any unused reagent can be refrozen, provided it hasn’t been exposed to the experimental process, thus ensuring optimal performance. -
Question:
What is the volume of the ProSignal Femto ECL Reagent kit?
Answer: The ProSignal Femto ECL Reagent kit contains 100ml, which is sufficient for multiple experiments. The generous volume allows for repeated use across various applications in your research. This ensures that labs can maximize the reagent's capabilities without frequently purchasing replacements, making it a cost-effective solution for extensive research projects. -
Question:
Can I use ProSignal Femto ECL Reagent for quantitative analysis?
Answer: Yes, ProSignal Femto ECL Reagent can be optimized for quantitative analysis of proteins in Western blotting. When used in conjunction with appropriate standards, it allows for precise quantification of protein levels within samples. This is especially beneficial in studies that require accurate protein measurement, such as pharmacokinetic studies or biomarker research in clinical settings. -
Question:
Is there a specific protocol for using ProSignal Femto ECL Reagent?
Answer: Using ProSignal Femto ECL Reagent involves a straightforward protocol that typically includes preparing the reagent, transferring samples to a membrane, and incubating with the reagent before detection. While specific protocols may vary depending on the experimental context, manufacturers generally provide detailed instructions to optimize performance. Following the protocol ensures reliable and reproducible results in your experiments. -
Question:
What are the key benefits of using ProSignal Femto ECL Reagent?
Answer: The primary benefits of using ProSignal Femto ECL Reagent include its ultra-high sensitivity, broad applicability across different sample types, and ease of use in standard Western blot protocols. Researchers commonly report enhanced visualization of low-abundance proteins, which is essential for accurate analysis. Furthermore, its consistency in performance enables studies to achieve reliable results across various experiments, thereby facilitating advancements in biological research. -
Question:
Does ProSignal Femto ECL Reagent have any specific safety considerations?
Answer: When using ProSignal Femto ECL Reagent, it’s important to observe standard laboratory safety protocols, such as wearing gloves and goggles. While the reagent is designed for laboratory use, researchers should consult the Safety Data Sheet (SDS) for detailed safety information and handling recommendations. This ensures a safe laboratory environment while maximizing the reagent's effectiveness. -
Question:
Where can I buy ProSignal Femto ECL Reagent, Low-femtogram, 1000cm2, 100ml Kit/Unit in Isle of Man?
Answer: You can purchase ProSignal Femto ECL Reagent from Ubuy in Isle of Man. Ubuy offers a reliable platform for sourcing laboratory reagents, ensuring you have access to quality products backed by customer support. Check their website to find the product you need and enjoy a seamless shopping experience.
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IMP 585
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Features & Benefits
- Attomole sensitivity for detecting low abundance proteins.
- 8+ hour quantitative signal for prolonged imaging.
- Ideal for both chemiluminescent and chemifluorescent applications.
- High signal-to-noise ratio for superior band quantification.
- Compatible with CCD imagers and traditional film.
- Unmatched performance compared to other ECL detection reagents.
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