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16340
QAZIPlasmid DNA Isolation Kit, MINIPREP 50Prep, P02-NIK01-50
Item #:
16340
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Product Details
- Certified QAZIPlasmid DNA Isolation Kit, MINIPREP 50Prep, P02-NIK01-50
- Allows quick and easy isolation of plasmid DNA from recombinant E. coli cultures
- Yields up to 15 µg of high-quality plasmid DNA in less than 30 minutes
- Utilizes silica-membrane based technology in a spin column or 96-well plate format
- Involves centrifugation, alkaline lysis, neutralization, and DNA retention steps
- Provides elution of the bound DNA in water or Tris-EDTA buffer
| Item Weight | 1.5 lbs (680 grams) |
Product Description
QAZIPlasmid DNA Isolation Kit, MINIPREP 50Prep, P02-NIK01-50
About This Item
Introducing the QAZIPlasmid DNA Isolation Kit - MINIPREP 50Prep (P02-NIK01-50) Looking for a quick and easy solution to isolate plasmid DNA from your recombinant E. coli cultures? Look no further than the QAZIPlasmid DNA Isolation Kit. This kit allows you to prepare high-quality plasmid DNA in less than 30 minutes, extracting up to 15 µg of DNA from just 1 to 5 ml of E.
coli culture. The QAZIPlasmid DNA Isolation Kit utilizes silica-membrane based technology in a convenient spin column or 96 well plate format. The isolation process involves three simple steps: bacterial harvesting, alkaline lysis, and neutralization using a chaotropic salt.
First, bacterial cells are harvested by centrifugation, ensuring optimal yield and purity of the DNA. Then, the alkaline lysis step breaks open the cells and releases the plasmid DNA. Neutralization with chaotropic salt stabilizes the DNA and prepares it for binding to the silica membrane.
The cleared lysate is then passed over the membrane, where the DNA binds to the silica due to adsorption in the presence of high salt conditions. After a couple of wash steps to remove impurities, the bound DNA is eluted in water or Tris-EDTA buffer, ready for downstream applications. The QAZIPlasmid DNA Isolation Kit is perfect for a variety of applications, including plasmid purification, DNA isolation, and plasmid DNA preparation. It comes with a comprehensive plasmid extraction protocol to guide you through each step, ensuring successful DNA isolation every time. Whether you are performing research, DNA sequencing, genetic engineering, or other molecular biology studies, the QAZIPlasmid DNA Isolation Kit provides a reliable and efficient method for obtaining high-quality plasmid DNA.
Don't settle for anything less than the best. Choose the QAZIPlasmid DNA Isolation Kit - MINIPREP 50Prep (P02-NIK01-50) and experience quick, easy, and reliable plasmid DNA extraction.
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Customer Questions & Answers
-
Question:
What is the QAZIPlasmid DNA Isolation Kit used for?
Answer: The QAZIPlasmid DNA Isolation Kit is designed for the efficient extraction of plasmid DNA from bacterial cultures. It utilizes a simple alkaline lysis method, allowing researchers to isolate high-quality plasmid DNA for a variety of applications. This can include cloning, sequencing, and transformation, making it a valuable tool in molecular biology laboratories and academic research settings. -
Question:
How many preparations does the QAZIPlasmid DNA Isolation Kit provide?
Answer: This kit is specifically formulated to perform up to 50 plasmid preparations. This high yield makes it ideal for labs conducting multiple experiments or for projects that require large amounts of plasmid DNA. Each preparation is designed to yield sufficient DNA for downstream applications, ensuring that researchers can rely on consistent results. -
Question:
Is the QAZIPlasmid DNA Isolation Kit suitable for all types of bacteria?
Answer: Yes, the QAZIPlasmid DNA Isolation Kit is versatile and can be used with a variety of bacterial strains, particularly those that are competent for transformation with plasmid DNA. It is an excellent choice for common laboratory strains such as E. coli. However, it is always advisable to verify the compatibility with your specific bacterial strain for optimal results. -
Question:
What are the main components included in the QAZIPlasmid DNA Isolation Kit?
Answer: The QAZIPlasmid DNA Isolation Kit includes several key components essential for plasmid extraction: Lysis Buffer, Neutralization Buffer, Wash Buffer, and Elution Buffer. Each component works in conjunction to lyse the bacterial cells, neutralize the lysate, wash away impurities, and finally elute the purified plasmid DNA, ensuring a high-purity product ready for your experimental needs. -
Question:
What is the recommended protocol for using the QAZIPlasmid DNA Isolation Kit?
Answer: The recommended protocol includes the steps of growing a bacterial culture, harvesting the cells, and then using the Lysis Buffer to break open the cells. Following neutralization and washing, the plasmid DNA is eluted using the provided Elution Buffer. Detailed instructions are typically included within the kit, which guides users through each step, ensuring efficient and reproducible results. -
Question:
What applications can be performed using the isolated plasmid DNA?
Answer: Isolated plasmid DNA from the QAZIPlasmid DNA Isolation Kit can be utilized in various applications, such as molecular cloning, transformation into competent cells, and performing PCR. It is also vital for sequencing procedures and construction of recombinant DNA, making it an essential part of genetic engineering and research in biotechnology. -
Question:
How long does the plasmid isolation process take with the QAZIPlasmid DNA Isolation Kit?
Answer: The entire plasmid isolation process generally takes about 30 to 60 minutes. This quick turnaround time is crucial for labs that need to conduct multiple experiments in a limited time frame. The straightforward steps involved mean that researchers can efficiently prepare their samples without significant downtime, which enhances productivity in research environments. -
Question:
Can the QAZIPlasmid DNA Isolation Kit be used for high-throughput applications?
Answer: Yes, the QAZIPlasmid DNA Isolation Kit is suitable for high-throughput applications, particularly due to its capacity for up to 50 preparations. This makes it an excellent choice for labs that require handling multiple samples simultaneously. Furthermore, adopting this kit can streamline workflows in environments focused on efficiency and reproducibility in molecular biology. -
Question:
What is the shelf life of the QAZIPlasmid DNA Isolation Kit?
Answer: The shelf life of the QAZIPlasmid DNA Isolation Kit is generally indicated on the packaging. Typically, if stored properly according to the provided instructions, the kit remains effective for several months. Always check the expiration date and manufacturer’s recommendations to ensure the reagents are functioning optimally for your plasmid isolation needs. -
Question:
Where can I buy the QAZIPlasmid DNA Isolation Kit in Isle of Man?
Answer: You can purchase the QAZIPlasmid DNA Isolation Kit from Ubuy in Isle of Man. Ubuy is a trusted online platform that offers a wide range of laboratory supplies, ensuring you can swiftly obtain this kit along with other essential products for your research work.
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IMP 65
This product is not Fulfilled by Ubuy and can take minimum 10 days in delivery. We might cancel the product from the order and refund you if any issue arise with the delivery of this product.
This item is not restrict in my country.(Please click on above link if this item is not restrict in your country, So our team will review and allow.)
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Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.
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Features & Benefits
- Offers quick and easy isolation of plasmid DNA
- Can prepare up to 15?g of high quality plasmid DNA in less than 30 minutes
- Utilizes silica-membrane based technology in a spin column or 96 well plate format
- Harvests bacteria by centrifugation, alkaline lysis, and neutralization
- Retains DNA using a membrane through adsorption onto silica in the presence of high salts
- Elutes DNA in water or Tris-EDTA buffer